Phosphoproteomics and morphology of stored human red blood cells treated by protein tyrosine phosphatases inhibitor.

ABSTRACT:

Abstract

The process of protein phosphorylation is involved in numerous cell functions. In particular, phosphotyrosine (pY) has been reported to play a role in red blood cell (RBC) functions, including the cytoskeleton organization. During their storage before transfusion, RBCs suffer from storage lesions that affect their energy metabolism and morphology. This study investigated the relationship between pY and the storage lesions. To do so, RBCs were treated (in the absence of calcium) with a protein tyrosine phosphatase inhibitor (orthovanadate [OV]) to stimulate phosphorylation and with 3 selective kinase inhibitors (KIs). Erythrocyte membrane proteins were studied by western blot analyses and phosphoproteomics (data are available via ProteomeXchange with identifier PXD039914) a

SUBMITTER: Bardyn M 

PROVIDER: S-EPMC10784683 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature