Requirement of GrgA for Chlamydia infectious progeny production, optimal growth, and efficient plasmid maintenance.

ABSTRACT:

Importance

Hallmarks of the developmental cycle of the obligate intracellular pathogenic bacterium Chlamydia are the primary differentiation of the infectious elementary body (EB) into the proliferative reticulate body (RB) and the secondary differentiation of RBs back into EBs. The mechanisms regulating these transitions remain unclear. In this report, we developed an effective novel strategy termed dependence on plasmid-mediated expression (DOPE) that allows for the knockdown of essential genes in Chlamydia. We demonstrate that GrgA, a Chlamydia-specific transcription factor, is essential for the secondary differentiation and optimal growth of RBs. We also show that GrgA, a chromosome-encoded regulatory protein, controls the maintenance of the chlamydial viru

SUBMITTER: Lu B 

PROVIDER: S-EPMC10790707 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature