Engineered bacteria titrate hydrogen sulfide and induce concentration-dependent effects on the host in a gut microphysiological system.

ABSTRACT: Hydrogen sulfide (H₂S) is a gaseous microbial metabolite whose role in gut diseases is debated, with contradictory results stemming from experimental difficulties associated with accurate dosing and measuring H₂S and the use of model systems that do not accurately represent the human gut environment. Here, we engineer Escherichia coli to titrate H₂S across the physiological range in a gut microphysiological system (chip) supportive of the co-culture of microbes and host cells. The chip is engineered to maintain H₂S gas tension and enables visualization of co-culture in real time with confocal microscopy. Engineered strains colonize the chip and are metabolically active for 2 days, during which they produce H₂S across a 16-fold range an

SUBMITTER: Hayes JA 

PROVIDER: S-EPMC10791167 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature