CRISPR/Cas13a-Assisted accurate and portable hepatitis D virus RNA detection.

ABSTRACT:

Background & aims

Hepatitis delta virus (HDV) infection accelerates the progression of chronic hepatitis B virus (HBV) infection, posing a large economic and health burden to patients. At present, there remains a lack of accurate and portable detection methods for HDV RNA. Here, we aim to establish a convenient, rapid, highly sensitive and specific method to detect HDV RNA using CRISPR-Cas13a technology.

Methods

We established fluorescence (F) and lateral flow strip (L) assays based on CRISPR-Cas13a combined with RT-PCR and RT-RAA for HDV RNA detection, respectively. we conducted a cohort study of 144 patients with HDV-IgG positive to evaluate the CRISPR-Cas13a diagnostic performance for identifying HDV in clinical samples, compared to RT-qPCR and RT-ddPCR.

Results

Fo

SUBMITTER: Tian Y 

PROVIDER: S-EPMC10796118 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature