SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome.

ABSTRACT: It is widely accepted that DNA replication fork stalling is a common occurrence during cell proliferation, but there are robust mechanisms to alleviate this and ensure DNA replication is completed prior to chromosome segregation. The SMC5/6 complex has consistently been implicated in the maintenance of replication fork integrity. However, the essential role of the SMC5/6 complex during DNA replication in mammalian cells has not been elucidated. In this study, we investigate the molecular consequences of SMC5/6 loss at the replication fork in mouse embryonic stem cells (mESCs), employing the auxin-inducible degron (AID) system to deplete SMC5 acutely and reversibly in the defined cellular contexts of replication fork stall and restart. In SMC5-depleted cells, we identify a defect in the res

SUBMITTER: Xu MJ 

PROVIDER: S-EPMC10815603 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature