Cloning, characterization, and spatio-temporal expression patterns of HdhSPARC and its responses to multiple stressors.

ABSTRACT: SPARC is an extracellular Ca²⁺-binding, secreted glycoprotein that plays a dynamic role in the growth and development of organisms. This study aimed to describe the isolation, characterization, and expression analysis of HdhSPARC in Pacific abalone (Haliotis discus hannai) to infer its potential functional role. The isolated HdhSPARC was 1633 bp long, encoding a polypeptide of 284 amino acid residues. Structurally, the SPARC protein in abalone is comprised of three biological domains. However, the structure of this protein varied between vertebrates and invertebrates, as suggested by their distinct clustering patterns in phylogenetic analysis. In early development, HdhSPARC was variably expressed, and higher expression was found in veliger larvae. Moreover, HdhSPARC was highly e

SUBMITTER: Hanif MA 

PROVIDER: S-EPMC10817941 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature