Characterization of CD4⁺ and CD8⁺ T cells responses in the mixed lymphocyte reaction by flow cytometry and single cell RNA sequencing.

ABSTRACT:

Background

The Mixed Lymphocyte Reaction (MLR) consists in the allogeneic co-culture of monocytes derived dendritic cells (MoDCs) with T cells from another donor. This in vitro assay is largely used for the assessment of immunotherapy compounds. Nevertheless, the phenotypic changes associated with lymphocyte responsiveness under MLR have never been thoroughly evaluated.

Methods

Here, we used multiplex cytokine and chemokine assays, multiparametric flow cytometry and single cell RNA sequencing to deeply characterize T cells activation and function in the context of CD4⁺- and CD8⁺-specific MLR kinetics.

Results

We showed that CD4⁺ and CD8⁺ T cells in MLR share common classical markers of response such as polyfunctionali

SUBMITTER: Mangelinck A 

PROVIDER: S-EPMC10820991 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature