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Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum.

ABSTRACT: This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of SPPE was 7.56 mg GAE/g and the polyphenol purity reached 70.5% after macroporous resin and Sephadex LH-20 purification. A total of 50 compounds were identified by UPLC-QTOF-MS/MS. The IC50 values of SPPE were 334.9 μg/mL, 6.290 μg /mL, 0.834 mg /mL and 0.6538 mg /mL for α-amylase, α-glucosidase, tyrosinase and AchE, respectively. Molecular docking technology further revealed the effects of SPPE on the above enzymes. This study provided information on the potential hypoglycemic, whitening and anti-Alzheimer's disease biological activities of SPPE, which had guiding significance for the purification and development of other seaweed polyphenols.

SUBMITTER: Jiang H

PROVIDER: S-EPMC10826943 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum.

Jiang Haiyun H Kong Li L Tang Hongguang H Wang Zhenzhen Z Liu Caiping C Zhang Jianhui J Chen Yuxin Y Shen Jinyang J Zhou Yue Y

PloS one 20240130 1

This study aimed to obtain a high yield and purity of Sargassum pallidum polyphenol extracts (SPPE) and study its enzyme activity. Fresh Sargassum pallidum seaweed was selected for optimization of ultrasound-assisted extraction (UAE) conditions and purification conditions using macroporous resin and Sephadex LH20 to obtain SPPE. The SPPE was characterized using UPLC-QTOF-MS/MS and α-amylase, α-glucosidase, tyrosinase, and AchE inhibitory activity were determined. The maximum extraction rate of S ...[more]

PMID: 38289914

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Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum.

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Study on the preparation and enzyme inhibitory activity of polyphenols from Sargassum pallidum.

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