Structural characterization and functional insights into the type II secretion system of the poly-extremophile Deinococcus radiodurans.

ABSTRACT: The extremophile bacterium D. radiodurans boasts a distinctive cell envelope characterized by the regular arrangement of three protein complexes. Among these, the Type II Secretion System (T2SS) stands out as a pivotal structural component. We used cryo-electron microscopy to reveal unique features, such as an unconventional protein belt (DR_1364) around the main secretin (GspD), and a cap (DR_0940) found to be a separated subunit rather than integrated with GspD. Furthermore, a novel region at the N-terminus of the GspD constitutes an additional second gate, supplementing the one typically found in the outer membrane region. This T2SS was found to contribute to envelope integrity, while also playing a role in nucleic acid and nutrient trafficking. Studies on intact cell envelopes show a c

SUBMITTER: Farci D 

PROVIDER: S-EPMC10828601 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature