A novel assay to measure low-density lipoproteins binding to proteoglycans.

ABSTRACT:

Background

The binding of low-density lipoprotein (LDL) to proteoglycans (PGs) in the extracellular matrix (ECM) of the arterial intima is a key initial step in the development of atherosclerosis. Although many techniques have been developed to assess this binding, most of the methods are labor-intensive and technically challenging to standardize across research laboratories. Thus, sensitive, and reproducible assay to detect LDL binding to PGs is needed to screen clinical populations for atherosclerosis risk.

Objectives

The aim of this study was to develop a quantitative, and reproducible assay to evaluate the affinity of LDL towards PGs and to replicate previously published results on LDL-PG binding.

Methods

Immunofluorescence microscopy was performed to visualize th

SUBMITTER: Geh EN 

PROVIDER: S-EPMC10830033 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature