CRISPR-TE: a web-based tool to generate single guide RNAs targeting transposable elements.

ABSTRACT:

Background

The CRISPR/Cas systems have emerged as powerful tools in genome engineering. Recent studies highlighting the crucial role of transposable elements (TEs) have stimulated research interest in manipulating these elements to understand their functions. However, designing single guide RNAs (sgRNAs) that are specific and efficient for TE manipulation is a significant challenge, given their sequence repetitiveness and high copy numbers. While various sgRNA design tools have been developed for gene editing, an optimized sgRNA designer for TE manipulation has yet to be established.

Results

We present CRISPR-TE, a web-based application featuring an accessible graphical user interface, available at https://www.crisprte.cn/ , and currently tailored to the human and mouse geno

SUBMITTER: Guo Y 

PROVIDER: S-EPMC10832116 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature