The chromatin-associated lncREST ensures effective replication stress response by promoting the assembly of fork signaling factors.

ABSTRACT: Besides the well-characterized protein network involved in the replication stress response, several regulatory RNAs have been shown to play a role in this critical process. However, it has remained elusive whether they act locally at the stressed forks. Here, by investigating the RNAs localizing on chromatin upon replication stress induced by hydroxyurea, we identified a set of lncRNAs upregulated in S-phase and controlled by stress transcription factors. Among them, we demonstrate that the previously uncharacterized lncRNA lncREST (long non-coding RNA REplication STress) is transcriptionally controlled by p53 and localizes at stressed replication forks. LncREST-depleted cells experience sustained replication fork progression and accumulate un-signaled DNA damage. Under replication stress,

SUBMITTER: Statello L 

PROVIDER: S-EPMC10834948 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature