Design and Engineering of Light-Induced Base Editors Facilitating Genome Editing with Enhanced Fidelity.

ABSTRACT: Base editors, which enable targeted locus nucleotide conversion in genomic DNA without double-stranded breaks, have been engineered as powerful tools for biotechnological and clinical applications. However, the application of base editors is limited by their off-target effects. Continuously expressed deaminases used for gene editing may lead to unwanted base alterations at unpredictable genomic locations. In the present study, blue-light-activated base editors (BLBEs) are engineered based on the distinct photoswitches magnets that can switch from a monomer to dimerization state in response to blue light. By fusing the N- and C-termini of split DNA deaminases with photoswitches Magnets, efficient A-to-G and C-to-T base editing is achieved in response to blue light in prokaryotic and eukaryo

SUBMITTER: Sun Y 

PROVIDER: S-EPMC10837352 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature