PQS and pyochelin in Pseudomonas aeruginosa share inner membrane transporters to mediate iron uptake.

ABSTRACT: Bacteria absorb different forms of iron through various channels to meet their needs. Our previous studies have shown that TseF, a type VI secretion system effector for Fe uptake, facilitates the delivery of outer membrane vesicle-associated Pseudomonas quinolone signal (PQS)-Fe³⁺ to bacterial cells by a process involving the Fe(III) pyochelin receptor FptA and the porin OprF. However, the form in which the PQS-Fe³⁺ complex enters the periplasm and how it is moved into the cytoplasm remain unclear. Here, we first demonstrate that the PQS-Fe³⁺ complex enters the cell directly through FptA or OprF. Next, we show that inner membrane transporters such as FptX, PchHI, and FepBCDG are not only necessary for Pseudomonas aeruginosa to absorb PQS-Fe

SUBMITTER: Zhang H 

PROVIDER: S-EPMC10846271 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature