Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer.

ABSTRACT: Cell-free RNA (cfRNA) allows assessment of health, status, and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases. Nevertheless, there is a lack of highly efficient and bias-free cfRNA isolation technologies due to the low abundance and instability of cfRNA. Here, we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids (containing cfRNA and viral RNA) in serum/plasma based on the inclusion of nucleic acids by metal-organic framework (MOF) materials, which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method. Importantly, the quality of cfRNA extracted by the MOF method is about 10-fold th

SUBMITTER: Sun Y 

PROVIDER: S-EPMC10860518 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature