Obscurin Maintains Myofiber Identity in Extraocular Muscles.

ABSTRACT:

Purpose

The cytoskeleton of the extraocular muscles (EOMs) is significantly different from that of other muscles. We aimed to investigate the role of obscurin, a fundamental cytoskeletal protein, in the EOMs.

Methods

The distribution of obscurin in human and zebrafish EOMs was compared using immunohistochemistry. The two obscurin genes in zebrafish, obscna and obscnb, were knocked out using CRISPR/Cas9, and the EOMs were investigated using immunohistochemistry, qPCR, and in situ hybridization. The optokinetic reflex (OKR) in five-day-old larvae and adult obscna-/-;obscnb-/- and sibling control zebrafish was analyzed. Swimming distance was recorded at the same age.

Results

The obscurin distribution pattern was similar in human and zebrafish EOMs. The proportion of slow

SUBMITTER: Kahsay A 

PROVIDER: S-EPMC10860686 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature