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αMI-domain of Integrin Mac-1 Binds the Cytokine Pleiotrophin Using Multiple Mechanisms.

ABSTRACT: The integrin Mac-1 (αMβ2, CD11b/CD18, CR3) is an important adhesion receptor expressed on macrophages and neutrophils. Mac-1 is also the most promiscuous member of the integrin family that binds a diverse set of ligands through its αMI-domain. However, the binding mechanism of most ligands is not clear. We have determined the interaction of αMI-domain with the cytokine pleiotrophin (PTN), a cationic protein known to bind αMI-domain and induce Mac-1-mediated cell adhesion and migration. Our data show that PTN's N-terminal domain binds a unique site near the N- and C-termini of the αMI-domain using a metal-independent mechanism. However, stronger interaction is achieved when an acidic amino acid in a zwitterionic motif in PTN's C-terminal domain chelates the divalent cation in the metal ion-dependent adhesion site of the active αMI-domain. These results indicate that αMI-domain can bind ligands using multiple mechanisms, and suggest that active αMI-domain prefers acidic amino acids in zwitterionic motifs.

SUBMITTER: Nguyen H 

PROVIDER: S-EPMC10862807 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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α<sub>M</sub>I-domain of Integrin Mac-1 Binds the Cytokine Pleiotrophin Using Multiple Mechanisms.

Nguyen Hoa H   Podolnikova Nataly P NP   Ugarova Tatiana P TP   Wang Xu X  

bioRxiv : the preprint server for biology 20240202

The integrin Mac-1 (α<sub>M</sub>β<sub>2</sub>, CD11b/CD18, CR3) is an important adhesion receptor expressed on macrophages and neutrophils. Mac-1 is also the most promiscuous member of the integrin family that binds a diverse set of ligands through its α<sub>M</sub>I-domain. However, the binding mechanism of most ligands is not clear. We have determined the interaction of α<sub>M</sub>I-domain with the cytokine pleiotrophin (PTN), a cationic protein known to bind α<sub>M</sub>I-domain and induc  ...[more]

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