α_MI-domain of Integrin Mac-1 Binds the Cytokine Pleiotrophin Using Multiple Mechanisms.

ABSTRACT: The integrin Mac-1 (α_Mβ₂, CD11b/CD18, CR3) is an important adhesion receptor expressed on macrophages and neutrophils. Mac-1 is also the most promiscuous member of the integrin family that binds a diverse set of ligands through its α_MI-domain. However, the binding mechanism of most ligands is not clear. We have determined the interaction of α_MI-domain with the cytokine pleiotrophin (PTN), a cationic protein known to bind α_MI-domain and induce Mac-1-mediated cell adhesion and migration. Our data show that PTN's N-terminal domain binds a unique site near the N- and C-termini of the α_MI-domain using a metal-independent mechanism. However, stronger interaction is achieved when an acidic amino acid in a zwitterionic motif in PTN'

SUBMITTER: Nguyen H 

PROVIDER: S-EPMC10862807 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature