An artificial nickel chlorinase based on the biotin-streptavidin technology.

ABSTRACT: Herein, we report on an artificial nickel chlorinase (ANCase) resulting from anchoring a biotinylated nickel-based cofactor within streptavidin (Sav). The resulting ANCase was efficient for the chlorination of diverse C(sp³)-H bonds. Guided by the X-ray analysis of the ANCase, the activity of the artificial chlorinase could be significantly improved. This approach opens interesting perspectives for late-stage functionalization of organic intermediates as it complements biocatalytic chlorination strategies.

SUBMITTER: Yu K 

PROVIDER: S-EPMC10863421 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature