Effects of Streptozotocin Induced Diabetes on One-Carbon Cycle and Sperm Function.

ABSTRACT:

Objective

Diabetic men suffer an increased risk of infertility associated with signs of oxidative damage and decreased methylation in sperm pointing to a deficit of the one-carbon cycle (1CC). We aimed to investigate this deficit using mice models (type 1 and 2) of streptozotocin-induced diabetes.

Materials and methods

In this experimental study, 50 male mice, aged eight weeks, were divided randomly into four groups: sham, control, type 1 diabetes mellitus (DM1), and DM2. The DM1 group was fed a normal diet (ND) for eight weeks, followed by five consecutive days of intraperitoneal administration of Streptozotocin (STZ, 50 mg/kg body weight). The DM2 group was fed a high-fat diet (HFD) for eight weeks, followed by a single intraperitoneal injection of STZ (100 mg/kg). After t

SUBMITTER: Pouriayevali F 

PROVIDER: S-EPMC10864770 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature