Large-Scale Preparation of Hair Follicle Germs Using a Microfluidic Device.

ABSTRACT: Hair follicle morphogenesis during embryonic development is driven by the formation of hair follicle germs (HFGs) via interactions between epithelial and mesenchymal cells. Bioengineered HFGs are potential tissue grafts for hair regenerative medicine because they can replicate interactions and hair follicle morphogenesis after transplantation. However, a mass preparation approach for HFGs is necessary for clinical applications, given that thousands of de novo hair follicles are required to improve the appearance of a single patient with alopecia. In this study, we developed a microfluidics-based approach for the large-scale preparation of HFGs. A simple flow-focusing microfluidic device allowed collagen solutions containing epithelial and mesenchymal cells to flow and generate collagen mic

SUBMITTER: Sugiyama E 

PROVIDER: S-EPMC10865290 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature