Identification of recG genetic interactions in Escherichia coli by transposon sequencing.
Ontology highlight
ABSTRACT: Importance
DNA damage and subsequent DNA repair processes are mutagenic in nature and an important driver of evolution in prokaryotes, including antibiotic resistance development. Genetic screening approaches, such as transposon sequencing (Tn-seq), have provided important new insights into gene function and genetic relationships. Here, we employed Tn-seq to gain insight into the function of the recG gene, which renders Escherichia coli cells moderately sensitive to a variety of DNA-damaging agents when they are absent. The reported recG genetic interactions can be used in combination with future screens to aid in a more complete reconstruction of DNA repair pathways in bacteria.
SUBMITTER: Bonde NJ
PROVIDER: S-EPMC10870727 | biostudies-literature | 2023 Dec
REPOSITORIES: biostudies-literature
Publications
Identification of <i>recG</i> genetic interactions in <i>Escherichia coli</i> by transposon sequencing.
Bonde Nina J NJ Wood Elizabeth A EA Myers Kevin S KS Place Michael M Keck James L JL Cox Michael M MM
Journal of bacteriology 20231129 12
<h4>Importance</h4>DNA damage and subsequent DNA repair processes are mutagenic in nature and an important driver of evolution in prokaryotes, including antibiotic resistance development. Genetic screening approaches, such as transposon sequencing (Tn-seq), have provided important new insights into gene function and genetic relationships. Here, we employed Tn-seq to gain insight into the function of the <i>recG</i> gene, which renders <i>Escherichia coli</i> cells moderately sensitive to a varie ...[more]
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