Dataset Information

Saccharomyces cerevisiae biofactory to produce naringenin using a systems biology approach and a bicistronic vector expression strategy in flavonoid production.

ABSTRACT:

Importance

Flavonoids are a group of compounds generally produced by plants with proven biological activity, which have recently beeen recommended for the treatment and prevention of diseases and ailments with diverse causes. In this study, naringenin was produced in adequate amounts in yeast after in silico design. The four genes of the involved enzymes from several organisms (bacteria and plants) were multi-expressed in two vectors carrying each two genes linked by a short viral peptide sequence. The batch kinetic behavior of the product, substrate, and biomass was described at lab scale. The engineered strain might be used in a more affordable and viable bioprocess for industrial naringenin procurement.

SUBMITTER: Mejia-Manzano LA

PROVIDER: S-EPMC10871697 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature

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<i>Saccharomyces cerevisiae</i> biofactory to produce naringenin using a systems biology approach and a bicistronic vector expression strategy in flavonoid production.

Mejía-Manzano Luis Alberto LA Ortiz-Alcaráz César Iván CI Parra Daza Laura E LE Suarez Medina Lina L Vargas-Cortez Teresa T Fernández-Niño Miguel M González Barrios Andrés Fernando AF González-Valdez José J

Microbiology spectrum 20231213 1

<h4>Importance</h4>Flavonoids are a group of compounds generally produced by plants with proven biological activity, which have recently beeen recommended for the treatment and prevention of diseases and ailments with diverse causes. In this study, naringenin was produced in adequate amounts in yeast after <i>in silico</i> design. The four genes of the involved enzymes from several organisms (bacteria and plants) were multi-expressed in two vectors carrying each two genes linked by a short viral ...[more]

PMID: 38088543

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