Nucleic acid hybridization-based detection of pathogenic RNA using microscale thermophoresis.

ABSTRACT: Infectious diseases are one of the world's leading causes of morbidity. Their rapid spread emphasizes the need for accurate and fast diagnostic methods for large-scale screening. Here, we describe a robust method for the detection of pathogens based on microscale thermophoresis (MST). The method involves the hybridization of a fluorescently labeled DNA probe to a target RNA and the assessment of thermophoretic migration of the resulting complex in solution within a 2 to 30-time window. We found that the thermophoretic migration of the nucleic acid-based probes is primarily determined by the fluorescent molecule used, rather than the nucleic acid sequence of the probe. Furthermore, a panel of uniformly labeled probes that bind to the same target RNA yields a more responsive detection patter

SUBMITTER: Avivi MY 

PROVIDER: S-EPMC10881438 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature