Project description:Interplay between herbal medicine and intestinal bacteria: interaction of willow bark extract and human intestinal microbiota in vitro
Project description:Willow bark is a byproduct from forestry and is obtained at an industrial scale. We upcycled this byproduct in a two-step procedure into sustainable electrode materials for symmetrical supercapacitors using organic electrolytes. The procedure employed precarbonization followed by carbonization using different types of KOH activation protocols. The obtained electrode materials had a hierarchically organized pore structure and featured a high specific surface area (>2500 m2 g-1) and pore volume (up to 1.48 cm3 g-1). The assembled supercapacitors exhibited capacitances up to 147 F g-1 in organic electrolytes concomitant with excellent cycling performance over 10,000 cycles at 0.6 A g-1 using coin cells. The best materials exhibited a capacity retention of 75% when changing scan rates from 2 to 100 mV s-1.
Project description:Novel birch bark dry extract (TE)-loaded polyvinyl alcohol (PVA) fiber mats intended for wound therapy were developed through an electrospinning process. Colloidal dispersions containing TE as the active substance were prepared by the high-pressure homogenization (HPH) technique using hydrogenated phospholipids as stabilizer. Subsequently, the colloidal dispersions were blended with aqueous PVA solutions in the ratio of 60:40 (wt.%) and electrospun to form the nanofiber mats. Fiber morphology examined using scanning electron microscopy (SEM) indicated that fibers were uniform and achieved diameters in the size range of 300-1586 nm. Confocal Raman spectral imaging gave good evidence that triterpenes were encapsulated within the electrospun mats. In vitro drug release and ex vivo permeation studies indicated that the electrospun nanofibers showed a sustained release of betulin, the main component of birch bark dry extract, making the examined dressings highly applicable for several wound care applications. Ex vivo wound healing studies proved that electrospun fiber mats containing TE accelerated wound healing significantly more than TE oleogel, which was comparable to an authorized product that consists of TE and sunflower oil and has proved to enhance wound healing. Therefore, our results conclude that the developed TE-PVA-based dressings show promising potential for wound therapy, an area where effective remedy is needed.
Project description:This study aimed to prepare microcrystalline cellulose (MCC) films with good mechanical properties via plasticization using a Chinese leek (CL, Allium tuberosum) extract. The microstructure, crystal structure, mechanical properties, barrier ability, and thermal properties of the films were investigated. The chemical structure analysis of CL extract showed the existence of cellulose, lignin, and low-molecular-weight substances, such as polysaccharides, pectins, and waxes, which could act as plasticizers to enhance the properties of MCC:CL biocomposite films. The results of scanning electron microscopy and atomic force microscopy analyses indicated the good compatibility between MCC and CL extract. When the volume ratio of MCC:CL was 7:3, the MCC:CL biocomposite film exhibited the best comprehensive performance in terms of water vapor permeability (2.11 × 10-10 g/m·s·Pa), elongation at break (13.2 ± 1.8%), and tensile strength (24.7 ± 2.5 MPa). The results of a UV absorption analysis demonstrated that the addition of CL extract improved the UV-shielding performance of the films. Therefore, this work not only proposes a facile method to prepare MCC films with excellent mechanical properties via plasticization using CL extract but also broadens the potential applications of MCC films in the packaging area.
Project description:Phellodendron amurense bark extract (Nexrutine®) has shown a favorable effect on prostate cancer in vivo and in vitro. We evaluated its tolerance in patients undergoing surgery or radiation for prostate cancer. Patients received Nexrutine® orally (500?mg tid) either 1 to 2 months preoperatively or 1 to 2?months prior to and with radiation therapy. Common Terminology Criteria for Adverse Events were used to measure tolerance. In total, 21 patients (9 surgery and 12 radiation) underwent treatment. During the Nexrutine® alone component, there were two transient grade 3 toxicities (hypokalemia and urinary incontinence). There was no grade 4 toxicity. For the combined Nexrutine® and radiation component, no additional patients suffered a grade 3 toxicity. All the toxicities were transient. By the end of the neoadjuvant treatment, 81% of the patients had a decline in prostate-specific antigen. This is the first report of patients with prostate cancer being treated with P. amurense bark extract, and it was very well tolerated. Toxicities were minimal and self-limited. This compound can be safely used in further evaluation of a treatment effect on cancer.
Project description:Corn fiber, a by-product of the starch industry, is presently incorporated in animal feed. However, it has arabinoxylans as added-value components (besides ferulic acid) that should be valorized. In this work, the raw material, a fraction enriched in arabinoxylans from corn fiber, previously produced by alkaline extraction from corn fiber and pre-concentrated by ultrafiltration, was further purified. The use of ultrafiltration operated in diafiltration mode (dia-ultrafiltration) was evaluated for the purification of the arabinoxylans fraction. The objective was to maximize the removal of the small contaminants from the fraction and to maximize the permeability and/or the permeate flux, by selecting the relevant operating conditions involved in this process. The removal of contaminants (%) was estimated when their apparent rejection stabilized. Edible films were produced, from the resultant purified arabinoxylans fraction, using glycerol as plasticizer (30% dry basis). Additionally, films with the incorporation of ferulic acid were developed, in order to obtain barriers with antioxidant activity. The films were characterized in terms of mechanical properties, antioxidant activity and permeability to water vapor. The films prepared presented a good potential to be used as packaging for food products with low water content.
Project description:Here, we report the fabrication and characterization of cellulose nanofiber (CNF)-based nanocomposite films reinforced with zinc oxide nanorods (ZnOs) and grapefruit seed extract (GSE). The CNF is isolated via a combination of chemical and physical methods, and the ZnO is prepared using a simple precipitation method. The ZnO and GSE are used as functional nanofillers to produce a CNF/ZnO/GSE film. Physical (morphology, chemical interactions, optical, mechanical, thermal stability, etc.) and functional (antimicrobial and antioxidant activities) film properties are tested. The incorporation of ZnO and GSE does not impact the crystalline structure, mechanical properties, or thermal stability of the CNF film. Nanocomposite films are highly transparent with improved ultraviolet blocking and vapor barrier properties. Moreover, the films exhibit effective antimicrobial and antioxidant actions. CNF/ZnO/GSE nanocomposite films with better quality and superior functional properties have many possibilities for active food packaging use.
Project description:Salix cortex-containing medicine is used against pain conditions, fever, headaches, and inflammation, which are partly mediated via arachidonic acid-derived prostaglandins (PGs). We used an activity-guided fractionation strategy, followed by structure elucidation experiments using LC-MS/MS, CD-spectroscopy, and 1D/2D NMR techniques, to identify the compounds relevant for the inhibition of PGE2 release from activated human peripheral blood mononuclear cells. Subsequent compound purification by means of preparative and semipreparative HPLC revealed 2'-O-acetylsalicortin (1), 3'-O-acetylsalicortin (2), 2'-O-acetylsalicin (3), 2',6'-O-diacetylsalicortin (4), lasiandrin (5), tremulacin (6), and cinnamrutinose A (7). In contrast to 3 and 7, compounds 1, 2, 4, 5, and 6 showed inhibitory activity against PGE2 release with different potencies. Polyphenols were not relevant for the bioactivity of the Salix extract but salicylates, which degrade to, e.g., catechol, salicylic acid, salicin, and/or 1-hydroxy-6-oxo-2-cycohexenecarboxylate. Inflammation presents an important therapeutic target for pharmacological interventions; thus, the identification of relevant key drugs in Salix could provide new prospects for the improvement and standardization of existing clinical medicine.
Project description:Bio-based polymer composites find increasing research and industrial interest in different areas of our life. In this study, cellulose nanofibers (CNFs) isolated from sugar beet pulp and nanoemulsion prepared from sugar beet pectin and pomegranate extract (PGE) were used for making films and used as coating with antioxidant and antimicrobial activities for paper. For Pectin/PGE nanoemulsion preparation, different ratios of PGE were mixed with pectin using ultrasonic treatment; the antibacterial properties were evaluated to choose the formula with the adequate antibacterial activity. The antioxidant activity of the nanoemulsion with the highest antimicrobial activity was also evaluated. The nanoemulsion with the optimum antibacterial activity was mixed with different ratios of CNFs. Mechanical, greaseproof, antioxidant activity, and antibacterial properties of the CNFs/Pectin/PGE films were evaluated. Finally, the CNFs/Pectin/PGE formulation with the highest antibacterial activity was tested as a coating material for paper. Mechanical, greaseproof, and air porosity properties, as well as water vapor permeability and migration of the coated layer from paper sheets in different media were evaluated. The results showed promising applicability of the CNFs/Pectin/PGE as films and coating material with antibacterial and antioxidant activities, as well as good stability for packaging aqueous, fatty, and acidic food products.
Project description:Cellulose nanocrystals (CNCs) not only have environmental protection characteristics of being lightweight, degradable, green, and renewable but also have some nanocharacteristics of high strength, large specific surface area, and obvious small size effect, so they are often used as a reinforcing agent in various polymers. However, the hydrogen bonding between CNC molecules is relatively strong, and they can easily aggregate and get entangled with each other. In this work, several large-porosity composite nanofiber films, KH550-CNC/waterborne polyurethane (WPU)/poly(vinyl alcohol) (PVAL) with KH550-modified CNCs, are prepared using poly(vinyl alcohol) (PVAL) solution and electrospinning technology. A variety of characterization methods are used to discuss and analyze the nanofiber materials, and the effects of the added amount of CNCs modified with KH550, spinning voltage, curing distance, and advancing speed on the morphology and performance of composite fibers are discussed separately. The results show that when the content of KH550-CNC is 1%, the composite fiber film obtained has the most regular morphology and the best spinnability, which is convenient for the specific application of fiber materials in a later period. In addition, the porosity of the obtained composite fiber film is 62.61%. Therefore, this work provides a theoretical basis and research strategy for the preparation of higher-porosity composite films as well as the development of new textile materials.