NLRP3 activation in macrophages promotes acute intestinal injury in neonatal necrotizing enterocolitis.

ABSTRACT:

Background

Macrophages are involved in various immune inflammatory disease conditions. This study aimed to investigate the role and mechanism of macrophages in regulating acute intestinal injury in neonatal necrotizing enterocolitis (NEC).

Methods

CD68, nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing 3 (NLRP3), cysteine aspartate-specific protease-1 (caspase-1), and interleukin-1β (IL-1β) in paraffin sections of intestinal tissues from NEC and control patients were detected with immunohistochemistry, immunofluorescence, and western blot. Hypertonic pet milk, hypoxia and cold stimulation were used to establish a mouse (wild type and Nlrp3^-/-) model of NEC. The mouse macrophage (RAW 264.7) and rat intestinal epithelial cel

SUBMITTER: Shi B 

PROVIDER: S-EPMC10884143 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature