Unconventional translation initiation factor EIF2A is required for Drosophila spermatogenesis.

ABSTRACT:

Background

EIF2A is an unconventional translation factor required for initiation of protein synthesis from non-AUG codons from a variety of transcripts, including oncogenes and stress related transcripts in mammalian cells. Its function in multicellular organisms has not been reported.

Results

Here, we identify and characterize mutant alleles of the CG7414 gene, which encodes the Drosophila EIF2A ortholog. We identified that CG7414 undergoes sex-specific splicing that regulates its male-specific expression. We characterized a Mi{Mic} transposon insertion that disrupts the coding regions of all predicted isoforms and is a likely null allele, and a PBac transposon insertion into an intron, which is a hypomorph. The Mi{Mic} allele is homozygous lethal, while the viable progeny

SUBMITTER: Lowe DD 

PROVIDER: S-EPMC10885012 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature