Advancing PEDV Vaccination: Comparison between Inactivated and Flagellin N-Terminus-Adjuvanted Subunit Vaccines.

ABSTRACT: Vaccinations can serve as an important preventive measure against the porcine epidemic diarrhea (PED) virus that currently threatens the swine industry. This study focuses on the development of a fusion protein vaccine, FliC₉₉-mCOE, which combines the N-terminus of flagellin (FliC₉₉) with a modified core neutralizing epitope (mCOE) of PEDV. In silico immunoinformatic analysis confirmed the construct's non-toxic, non-allergenic, and highly antigenic nature. Molecular docking and molecular dynamics (MD) simulations demonstrated FliC₉₉-mCOE's strong binding to the TLR-5 immunological receptor. Repeated exposure simulations and immunological simulations suggested enhanced cell-mediated immunity. Both FliC₉₉-mCOE and an ina

SUBMITTER: Murtaza A 

PROVIDER: S-EPMC10892538 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature