Mtb HLA-E-tetramer-sorted CD8⁺ T cells have a diverse TCR repertoire.

ABSTRACT: HLA-E molecules can present self- and pathogen-derived peptides to both natural killer (NK) cells and T cells. T cells that recognize HLA-E peptides via their T cell receptor (TCR) are termed donor-unrestricted T cells due to restricted allelic variation of HLA-E. The composition and repertoire of HLA-E TCRs is not known so far. We performed TCR sequencing on CD8⁺ T cells from 21 individuals recognizing HLA-E tetramers (TMs) folded with two Mtb-HLA-E-restricted peptides. We sorted HLA-E Mtb TM⁺ and TM^- CD8⁺ T cells directly ex vivo and performed bulk RNA-sequencing and single-cell TCR sequencing. The identified TCR repertoire was diverse and showed no conservation between and within individuals. TCRs selected from our single-

SUBMITTER: Voogd L 

PROVIDER: S-EPMC10909886 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature