Alzheimer's disease-induced phagocytic microglia express a specific profile of coding and non-coding RNAs.

ABSTRACT:

Introduction

Alzheimer's disease (AD) is a neurodegenerative disease and the main cause of dementia in the elderly. AD pathology is characterized by accumulation of microglia around the beta-amyloid (Aβ) plaques which assumes disease-specific transcriptional signatures, as for the disease-associated microglia (DAM). However, the regulators of microglial phagocytosis are still unknown.

Methods

We isolated Aβ-laden microglia from the brain of 5xFAD mice for RNA sequencing to characterize the transcriptional signature in phagocytic microglia and to identify the key non-coding RNAs capable of regulating microglial phagocytosis. Through spatial sequencing, we show the transcriptional changes of microglia in the AD mouse brain in relation to Aβ proximity.

Results

Finally, w

SUBMITTER: Scoyni F 

PROVIDER: S-EPMC10916983 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature