Influence of plasma collection tubes on N-glycome in human blood samples.

ABSTRACT:

Background and aims

Quantitative analysis of plasma N-glycome is a promising method for identifying disease biomarkers. This study aimed to investigate the impact of using blood collection tubes with different anticoagulants on plasma N-glycome.

Materials and methods

We used a robust mass spectrometry method to profile plasma N-glycomes in two cohorts of healthy volunteers (cohort 1, n = 16; cohort 2, n = 53). The influence of three commonly used blood collection tubes on fully characterized N-glycomic profiles were explored.

Results

Principal component analysis revealed distinct clustering of blood samples based on the collection tubes. Pairwise comparisons demonstrated significant differences between EDTA and heparin plasma in 55 out of 82 quantified N-glycan traits

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC10924059 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature