Ontology highlight
ABSTRACT:
SUBMITTER: Cao R
PROVIDER: S-EPMC10925295 | biostudies-literature | 2024 Mar
REPOSITORIES: biostudies-literature
Cao Ruili R Gozlan Oren O Tveriakhina Lena L Zhou Haixia H Jiang Hanjie H Cole Philip A PA Aster Jon C JC Sprinzak David D Blacklow Stephen C SC
bioRxiv : the preprint server for biology 20240303
Mind bomb 1 (MIB1) is a RING E3 ligase that ubiquitinates Notch ligands, a necessary step for induction of Notch signaling. The structural basis for binding of the JAG1 ligand by the N-terminal region of MIB1 is known, yet how the ankyrin (ANK) and RING domains of MIB1 cooperate to catalyze ubiquitin transfer from E2~Ub to Notch ligands remains unclear. Here, we show that the third RING domain and adjacent coiled coil region of MIB1 (ccRING3) drives MIB1 dimerization and that ubiquitin transfer ...[more]