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Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia.

ABSTRACT: Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic diets comprising equivalent amounts of the protein supply derived from either PLANT, ANIMAL, INSECT, or BACTERIAL (bacterial biomass) sources were fed to triplicate groups of fish (IBW: 12 g) for 46 days. Fish fed the ANIMAL and BACTERIAL diets showed significantly higher weight gains than those fed the PLANT and INSECT diets (p < 0.05). Relative abundance at the phylum level showed that Bacteroidetes, Fusobacteria, and Proteobacteria were the more abundant phyla in tilapia's intestine, while Cetobacterium was the most representative genus in all treatments. Interesting patterns were observed in the correlation between amino acid intake and genus and species abundance. Metabolism prediction analysis showed that BACTERIAL amine and polyamine degradation pathways are modulated depending on diets. In conclusion, different protein sources modulate the relationship between bacteria functional pathways and amino acid intake.

SUBMITTER: do Vale Pereira G

PROVIDER: S-EPMC10930938 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia.

do Vale Pereira Gabriella G Teixeira Carla C Couto José J Dias Jorge J Rema Paulo P Gonçalves Ana Teresa AT

Animals : an open access journal from MDPI 20240224 5

Dietary protein quality plays a key role in maintaining intestinal mucosal integrity, but also modulates the growth of luminal microorganisms. This work assessed the effect of dietary protein sources on the performance, gut morphology, and microbiome in Nile tilapia. Four isonitrogenous and isolipidic diets comprising equivalent amounts of the protein supply derived from either PLANT, ANIMAL, INSECT, or BACTERIAL (bacterial biomass) sources were fed to triplicate groups of fish (IBW: 12 g) for 4 ...[more]

PMID: 38473099

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Project description:IntroductionExtreme water temperature affects the well-being of all aquatic animals, including fish. Higher temperatures can lead to the generation of reactive oxygen species (ROS), which can induce oxidative stress and negatively impact fish health and well-being. This study investigated the protective effects of seaweed extract on growth, antioxidant status, inflammatory responses, and gut microbiota to gain a better understanding of the acclimatization ability of Nile tilapia, Oreochromis niloticus in response to oxidative stress caused by high water temperatures.MethodsRed-seaweed, Gracilaria tenuistipitata rich in polyphenols (i.e., total phenolics and flavonoids content) was considered for the preparation of the Gracilaria extract (GE) for the study. Nile tilapia were fed the GE supplemented diet along with a control diet for 42 days, followed by 14 days of temperature ramping at a rate of 1°C every two days to the desired target (35°C) and 14 days of holding at 32°C for acclimatation.ResultsNile tilapia fed the GE had a significantly higher growth performance attributed to increased muscle fiber size compared to control (p < 0.05) after the 70 days of feeding trial. Fish fed the GE diet also showed a significantly lower lipid peroxidation by decreased malondialdehyde level when compared to control (p < 0.05). Furthermore, GE diet exhibited increased red blood cell counts with the decreased number of cellular and nuclear abnormalities. The gene expression of tight junction (i.e., occludin, claudin1, ZO-1) and nrf2 (antioxidant biomarker) were upregulated, while hsp70 (related to stress response) was downregulated in fish fed the GE diet. Additionally, GE supplementation led to an increase in bacterial diversity and the abundance of phylum Firmicutes, order Lactobacillales, and genera Sphingobacterium and Prevotella in the distal gut of Nile tilapia, which are mostly considered as beneficial for fish.ConclusionThe findings suggest that GE has the potential to be used as a dietary supplement to improve health, particularly as a stress-resistant supplement in the diet for Nile tilapia. This study may help make more informed decisions for tailoring the nutrient requirements of fish in the face of climate warming.

Project description:Backgrounds and aimsAluminum contamination of water is becoming increasingly serious and threatens the health status of fish. Lactobacillus plantarum CCFM639 was previously shown to be a potential probiotic for alleviation aluminum toxicity in Nile tilapia. Considering the significant role of the gut microbiota on fish health, it seems appropriate to explore the relationships among aluminum exposure, probiotic supplementation, and the gut microbiota in Nile tilapia and to determine whether regulation of the gut microbiota is related to alleviation of aluminum toxicity by a probiotic in Nile tilapia.Methods and resultsThe tilapia were assigned into four groups, control, CCFM639 only, aluminum only, and aluminum + CCFM639 groups for an experimental period of 4 weeks. The tilapia in the aluminum only group were grown in water with an aluminum ion concentration of 2.73 mg/L. The final concentration of CCFM639 in the diet was 108 CFU/g. The results show that environmental aluminum exposure reduced the numbers of L. plantarum in tilapia feces and altered the gut microbiota. As the predominant bacterial phyla in the gut, the abundances of Bacteroidetes and Proteobacteria in aluminum-exposed fish were significantly elevated and lowered, respectively. At the genus level, fish exposed to aluminum had a significantly lower abundance of Deefgea, Plesiomonas, and Pseudomonas and a greater abundance of Flavobacterium, Enterovibrio, Porphyromonadaceae uncultured, and Comamonadaceae. When tilapia were exposed to aluminum, the administration of a probiotic promoted aluminum excretion through the feces and led to a decrease in the abundance of Comamonadaceae, Enterovibrio and Porphyromonadaceae. Notably, supplementation with a probiotic only greatly decreased the abundance of Aeromonas and Pseudomonas.ConclusionAluminum exposure altered the diversity of the gut microbiota in Nile tilapia, and probiotic supplementation allowed the recovery of some of the diversity. Therefore, regulation of gut microbiota with a probiotic is a possible mechanism for the alleviation of aluminum toxicity in Nile tilapia.

Project description:BackgroundFish gut microbial colonisation starts during larval stage and plays an important role in host's growth and health. To what extent first colonisation could influence the gut microbiome succession and growth in later life remains unknown. In this study, Nile tilapia embryos were incubated in two different environments, a flow-through system (FTS) and a biofloc system (BFS); hatched larvae were subsequently cultured in the systems for 14 days of feeding (dof). Fish were then transferred to one common recirculating aquaculture system (RAS1, common garden, 15-62 dof), followed by a growth trial in another RAS (RAS2, growth trial, 63-105 dof). In RAS2, fish were fed with two types of diet, differing in non-starch polysaccharide content. Our aim was to test the effect of rearing environment on the gut microbiome development, nutrient digestibility and growth performance of Nile tilapia during post-larvae stages.ResultsLarvae cultured in the BFS showed better growth and different gut microbiome, compared to FTS. After the common garden, the gut microbiome still showed differences in species composition, while body weight was similar. Long-term effects of early life rearing history on fish gut microbiome composition, nutrient digestibility, nitrogen and energy balances were not observed. Still, BFS-reared fish had more gut microbial interactions than FTS-reared fish. A temporal effect was observed in gut microbiome succession during fish development, although a distinct number of core microbiome remained present throughout the experimental period.ConclusionOur results indicated that the legacy effect of first microbial colonisation of the fish gut gradually disappeared during host development, with no differences in gut microbiome composition and growth performance observed in later life after culture in a common environment. However, early life exposure of larvae to biofloc consistently increased the microbial interactions in the gut of juvenile Nile tilapia and might possibly benefit gut health.

Dataset Information

Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia.

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Dietary Protein Quality Affects the Interplay between Gut Microbiota and Host Performance in Nile Tilapia.

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