A new method to experimentally quantify dynamics of initial protein-protein interactions.

ABSTRACT: Cells run on initiation of protein-protein interactions, which are dynamically tuned spatially and temporally to modulate cellular events. This tuning can be physical, such as attaching the protein to a cargo or protein complex, thereby altering its diffusive properties, or modulating the distance between protein pairs, or chemical, by altering the proteins' conformations (e.g., nucleotide binding state of an enzyme, post-translational modification of a protein, etc.). Because a dynamic and changing subset of proteins in the cell could be in any specific state, ensemble measurements are not ideal-to untangle which of the factors are important, and how, we need single-molecule measurements. Experimentally, until now we have not had good tools to precisely measure initiation of such protein-

SUBMITTER: Janakaloti Narayanareddy BR 

PROVIDER: S-EPMC10933273 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature