A robust, scalable, and cost-efficient approach to whole genome sequencing of RSV directly from clinical samples.

ABSTRACT: Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infections causing significant morbidity and mortality among children and the elderly; two RSV vaccines and a monoclonal antibody have recently been approved. Thus, there is an increasing need for a detailed and continuous genomic surveillance of RSV circulating in resource-rich and resource-limited settings worldwide. However, robust, cost-effective methods for whole genome sequencing of RSV from clinical samples that are amenable to high-throughput are still scarce. We developed Next-RSV-SEQ, an experimental and computational pipeline to generate whole genome sequences of historic and current RSV genotypes by in-solution hybridization capture-based next generation sequencing. We optimized this workflow

SUBMITTER: Kondgen S 

PROVIDER: S-EPMC10935636 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature