Fibroblasts facilitate lymphatic vessel formation in transplanted heart.

ABSTRACT: Rationale: Lymphangiogenesis plays a critical role in the transplanted heart. The remodeling of lymphatics in the transplanted heart and the source of newly formed lymphatic vessels are still controversial, especially the mechanism of lymphangiogenesis remains limited. Methods: Heart transplantation was performed among BALB/c, C57BL/6J, Cag-Cre, Lyve1-CreER^T2;Rosa26-tdTomato and Postn(2A-CreER^T2-wpre-pA)1;Rosa26-DTA mice. scRNA-seq, Elisa assay, Western blotting, Q-PCR and immunohistochemical staining were used to identify the cells and cell-cell communications of allograft heart. Cell depletion was applied to in vivo and in vitro experiments. Whole-mount staining and three-dime

SUBMITTER: Gong H 

PROVIDER: S-EPMC10945330 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature