The medulla controls effector primed γδT-cell development in the adult mouse thymus.

ABSTRACT: γδT cells are produced in the thymus throughout life and provide immunity at epithelial-rich sites. Unlike conventional αβT cells, γδT-cell development involves intrathymic acquisition of effector function, with priming for either IL17 or IFN-γ production occurring during embryonic or adult life, respectively. How the thymus controls effector-primed γδT-cell generation in adulthood is poorly understood. Here, we distinguished de novo γδT cells from those undergoing thymus recirculation and/or retention using Rag2GFP mice alongside markers of maturation/effector priming including CD24, CD25, CD73, and IFN-γ, the latter by crossing with IFN-γ^YFP GREAT mice. We categorize newly developing γδT-cells into an ordered sequence where CD25⁺ CD73^- IFN-γ^YFP-

SUBMITTER: James KD 

PROVIDER: S-EPMC10947249 | biostudies-literature | 2023 Jun

REPOSITORIES: biostudies-literature