Circular RNA hsa_circ_0005519 contributes to acute kidney injury via sponging microRNA-98-5p.

ABSTRACT:

Background

This study intends to explore the role and molecular mechanism of hsa_circ_0005519 in acute kidney injury (AKI).

Methods

We conducted reverse transcription-qPCR for human serum to determine levels of hsa_circ_0005519 in AKI patients and healthy controls. Hsa_circ_0005519 was inhibited for expression in HK-2 cells using specific siRNAs. A number of techniques, MTT and ELISA assays, were used to analyze the potential role of hsa_circ_0005519 in cell viability, oxidative stress, and inflammation of LPS-induced HK-2 cells.

Results

The serum of patients with AKI exhibited a significant increase in hsa_circ_0005519 expression, compared with healthy controls. Hsa_circ_0005519 was knockdown by siRNA, and its knockdown led to cell viability increase in LPS-induced H

SUBMITTER: Jiang L 

PROVIDER: S-EPMC10949765 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature