COL0RME: Super-resolution microscopy based on sparse blinking/fluctuating fluorophore localization and intensity estimation.

ABSTRACT: To overcome the physical barriers caused by light diffraction, super-resolution techniques are often applied in fluorescence microscopy. State-of-the-art approaches require specific and often demanding acquisition conditions to achieve adequate levels of both spatial and temporal resolution. Analyzing the stochastic fluctuations of the fluorescent molecules provides a solution to the aforementioned limitations, as sufficiently high spatio-temporal resolution for live-cell imaging can be achieved using common microscopes and conventional fluorescent dyes. Based on this idea, we present COL0RME, a method for covariance-based super-resolution microscopy with intensity estimation, which achieves good spatio-temporal resolution by solving a sparse optimization problem in the covariance domain a

SUBMITTER: Stergiopoulou V 

PROVIDER: S-EPMC10951805 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature