Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy.

ABSTRACT: Protein labeling through transient and repetitive hybridization of short, fluorophore-labeled DNA oligonucleotides has become widely applied in various optical super-resolution microscopy methods. The main advantages are multitarget imaging and molecular quantification. A challenge is the high background signal originating from the presence of unbound fluorophore-DNA labels in solution. Here, we report the self-quenching of fluorophore dimers conjugated to DNA oligonucleotides as a general concept to reduce the fluorescence background. Upon hybridization, the fluorescence signals of both fluorophores are restored. We expand the toolbox of fluorophores suitable for self-quenching and report their spectra and hybridization equilibria. We apply self-quenched fluorophore-DNA labels to stimulat

SUBMITTER: Kessler LF 

PROVIDER: S-EPMC11264260 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature