Dataset Information
Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study.
Ontology highlight
ABSTRACT: Background and purpose
Many sectors use nanoparticles and dispose of them in the aquatic environment without deciding the fate of these particles.Experimental approach
To identify a benign species of nanoparticles which can cause minimum harm to the aquatic environment, a comparative study was done with chemically synthesized silver nanoparticles (AgNPs) and green tea mediated synthesis (GT/AgNP) in both in vitro using human alveolar cancer cell line (A549) and normal cell line (L132), and in in vivo with zebrafish embryos.Key results
The in vitro studies revealed that GT/AgNPs were less toxic to normal cells than cancer cells. The GT/AgNPs showed high biocompatibility for zebrafish embryos monitored microscopically for their developmental stages and by cumulative hatchability studies. The reduced hatchability found in the AgNPs-treated group was correlated by differential gene expression of zebrafish hatching enzymes (ZHE) (ZHE1 and ZHE2).Conclusion
The results indicated that nanoparticles can affect the hatching of zebrafish embryos and elicit toxicity at the gene level.
SUBMITTER: R SD
PROVIDER: S-EPMC10974822 | biostudies-literature | 2024
REPOSITORIES: biostudies-literature
Publications
Beneficial effects of bioinspired silver nanoparticles on zebrafish embryos including a gene expression study.
ADMET & DMPK 20240101 1
<h4>Background and purpose</h4>Many sectors use nanoparticles and dispose of them in the aquatic environment without deciding the fate of these particles.<h4>Experimental approach</h4>To identify a benign species of nanoparticles which can cause minimum harm to the aquatic environment, a comparative study was done with chemically synthesized silver nanoparticles (AgNPs) and green tea mediated synthesis (GT/AgNP) in both in vitro using human alveolar cancer cell line (A549) and normal cell line ( ...[more]
