The NADPH oxidase 2 subunit p47^phox binds to the WAVE regulatory complex and p22^phox in a mutually exclusive manner.

ABSTRACT: The actin cytoskeleton and reactive oxygen species (ROS) both play crucial roles in various cellular processes. Previous research indicated a direct interaction between two key components of these systems: the WAVE1 subunit of the WAVE regulatory complex (WRC), which promotes actin polymerization and the p47^phox subunit of the NADPH oxidase 2 complex (NOX2), which produces ROS. Here, using carefully characterized recombinant proteins, we find that activated p47^phox uses its dual Src homology 3 domains to bind to multiple regions within the WAVE1 and Abi2 subunits of the WRC, without altering WRC's activity in promoting Arp2/3-mediated actin polymerization. Notably, contrary to previous findings, p47^phox uses the same binding pocket to interact with both the

SUBMITTER: Kuihon SVNP 

PROVIDER: S-EPMC10979099 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature