Oligomeric organization of membrane proteins from native membranes at nanoscale spatial and single-molecule resolution.

ABSTRACT: The oligomeric organization of membrane proteins in native cell membranes is a critical regulator of their function. High-resolution quantitative measurements of oligomeric assemblies and how they change under different conditions are indispensable to understanding membrane protein biology. We report Native-nanoBleach, a total internal reflection fluorescence microscopy-based single-molecule photobleaching step analysis technique to determine the oligomeric distribution of membrane proteins directly from native membranes at an effective spatial resolution of ~10 nm. We achieved this by capturing target membrane proteins in native nanodiscs with their proximal native membrane environment using amphipathic copolymers. We applied Native-nanoBleach to quantify the oligomerization status of str

SUBMITTER: Walker G 

PROVIDER: S-EPMC10981947 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature