Insights into the nuclear and mitochondrial genome of the Lemon shark <i>Negaprion brevirostris</i> using low-coverage sequencing: Genome size, repetitive elements, mitochondrial genome, and phylogenetic placement.

ABSTRACT: The Lemon shark Negaprion brevirostris is an important species experiencing conservation issues that is in need of genomic resources. Herein, we conducted a genome survey sequencing in N. brevirostris and determined genome size, explored repetitive elements, assembled and annotated the 45S rRNA DNA operon, and assembled and described in detail the mitochondrial genome. Lastly, the phylogenetic position of N. brevirostris in the family Carcharhinidae was examined using translated protein coding genes. The estimated haploid genome size ranged between 2.29 and 2.58 Gbp using a k-mer analysis, which is slightly below the genome size estimated for other sharks belonging to the family Carcharhinidae. Using a k-mer analysis, approx. 64-71 % of the genome of N. brevirostris

SUBMITTER: Baeza JA 

PROVIDER: S-EPMC10990291 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature