Salt-tolerant and thermostable mechanisms of an endoglucanase from marine Aspergillus niger.

ABSTRACT: The cellulase cocktail of marine Aspergillus niger exhibited salt-tolerant and thermostable properties, which is of great potential in industrial application. In order to excavate the single tolerant cellulase components from complex cellulase cocktail, constitutive homologous expression was employed for direct obtainment of the endoglucanase (AnEGL). Enzymatic property study revealed that AnEGL exhibited a property of salt tolerance and a strong thermostability in high salinity environment. Significantly, its activity increased to 129% and the half-life at 65 °C increased to 27.7-fold with the presence of 4.5 M NaCl. Molecular dynamics simulation revealed that Na⁺ and Cl^- could form salt bridges with charged residues, and then influenced the activity of loops and the

SUBMITTER: Cai LN 

PROVIDER: S-EPMC10991132 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature