AlphaFold-assisted structure determination of a bacterial protein of unknown function using X-ray and electron crystallography.

ABSTRACT: Macromolecular crystallography generally requires the recovery of missing phase information from diffraction data to reconstruct an electron-density map of the crystallized molecule. Most recent structures have been solved using molecular replacement as a phasing method, requiring an a priori structure that is closely related to the target protein to serve as a search model; when no such search model exists, molecular replacement is not possible. New advances in computational machine-learning methods, however, have resulted in major advances in protein structure predictions from sequence information. Methods that generate predicted structural models of sufficient accuracy provide a powerful approach to molecular replacement. Taking advantage of these advances, AlphaFold predictions were ap

SUBMITTER: Miller JE 

PROVIDER: S-EPMC10994174 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature