Identification of host proteins interacting with the E protein of porcine epidemic diarrhea virus.

ABSTRACT:

Introduction

Porcine epidemic diarrhea (PED) is an acute, highly contagious, and high-mortality enterophilic infectious disease caused by the porcine epidemic diarrhea virus (PEDV). PEDV is globally endemic and causes substantial economic losses in the swine industry. The PEDV E protein is the smallest structural protein with high expression levels that interacts with the M protein and participates in virus assembly. However, how the host proteins interact with E proteins in PEDV replication remains unknown.

Methods

We identified host proteins that interact with the PEDV E protein using a combination of PEDV E protein-labeled antibody co-immunoprecipitation and tandem liquid-chromatography mass-spectroscopy (LC-MS/MS).

Results

Bioinformatical analysis showed that in e

SUBMITTER: Qiu Y 

PROVIDER: S-EPMC10994376 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature