Indocyanine Green-Labeled Platelets for Survival and Recovery Studies.

ABSTRACT:

Introduction

Before being implemented in daily clinical routine, new production strategies for platelet concentrates (PCs) must be validated for their efficacy. Besides in vitro testing, the establishment of new methods requires the labeling of platelets for in vivo studies of platelets' survival and recovery. Indocyanine green (ICG) is a Food and Drug Administration-approved near-infrared (NIR) fluorescent dye for diagnostic use in vivo, suitable for non-radioactive direct cell labeling of platelets.

Methods

Platelets from PCs in storage solutions with different plasma concentrations were labeled with ICG up to concentrations of 200 μm. Whole blood (WB) was used as an ex vivo matrix to monitor the labeling stability of ICG-labeled platelets. The impact of labeling processes

SUBMITTER: von Behren JM 

PROVIDER: S-EPMC10996059 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature