An effective strategy for assembling the sex-limited chromosome.

ABSTRACT:

Background

Most currently available reference genomes lack the sequence map of sex-limited (such as Y and W) chromosomes, which results in incomplete assemblies that hinder further research on sex chromosomes. Recent advancements in long-read sequencing and population sequencing have provided the opportunity to assemble sex-limited chromosomes without the traditional complicated experimental efforts.

Findings

We introduce the first computational method, Sorting long Reads of Y or other sex-limited chromosome (SRY), which achieves improved assembly results compared to flow sorting. Specifically, SRY outperforms in the heterochromatic region and demonstrates comparable performance in other regions. Furthermore, SRY enhances the capabilities of the hybrid assembly software, res

SUBMITTER: Wang XB 

PROVIDER: S-EPMC11020242 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature