Development of a genetic system for Haloferax gibbonsii LR2-5, model host for haloarchaeal viruses.

ABSTRACT: Archaeal viruses are among the most enigmatic members of the virosphere, and their diverse morphologies raise many questions about their infection mechanisms. The study of molecular mechanisms underlying virus-host interactions hinges upon robust model organisms with a system for gene expression and deletion. Currently, there are only a limited number of archaea that have associated viruses and have a well-developed genetic system. Here, we report the development of a genetic system for the euryarchaeon Haloferax gibbonsii LR2-5. This strain can be infected by multiple viruses and is a model for the study of virus-host interactions. We created a Hfx. gibbonsii LR2-5 ∆pyrE strain, resulting in uracil auxotrophy, which could be used as a selection marker. An expression p

SUBMITTER: Tittes C 

PROVIDER: S-EPMC11022537 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature