Novel monoclonal antibodies against house dust mite allergen Der p 21 and their application to analyze allergen extracts.

ABSTRACT:

Background

Allergen extracts and recombinant allergens are used in allergy diagnostics and immunotherapy. Since allergen extracts from different manufacturers lack proper standardization regarding their composition, monoclonal antibodies (MAbs) against specific allergen components can be used for their identification and quantification in allergen extracts. This study aimed to generate MAbs against allergen Der p 21 of Dermatophagoides pteronyssinus for the analysis of allergen extracts.

Methods

Recombinant Der p 21 was expressed in E. coli and purified using affinity chromatography. MAbs against Der p 21 were generated using hybridoma technology. House dust mite (HDM) allergen extracts were analyzed using the newly developed sandwich enzyme-linked immunosorben

SUBMITTER: Rudokas V 

PROVIDER: S-EPMC11032652 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature